{"id":34265,"date":"2021-06-24T16:51:23","date_gmt":"2021-06-24T14:51:23","guid":{"rendered":"https:\/\/genomtec.com\/?p=34265"},"modified":"2021-06-25T11:14:06","modified_gmt":"2021-06-25T09:14:06","slug":"genetic-tests-genomtec-sars-cov-2-duo-kit-cat-no-ga00b-and-direct-rt-lamp-kit-cat-no-ga00c-detect-multiple-virus-lineages","status":"publish","type":"post","link":"https:\/\/genomtec.com\/en\/2021\/06\/24\/genetic-tests-genomtec-sars-cov-2-duo-kit-cat-no-ga00b-and-direct-rt-lamp-kit-cat-no-ga00c-detect-multiple-virus-lineages\/","title":{"rendered":"Genetic tests Genomtec SARS-CoV-2 Duo Kit (Cat. no.: GA00B) and Direct-RT-LAMP Kit (Cat. no.: GA00C) detect multiple virus lineages"},"content":{"rendered":"<p><\/p>\r\n<p>RT-LAMP Duo and Direct Kits\u2019 reliability in recognition of identified SARS-CoV-2 significant variants, among them the Breton that has the potential to escape standard RT-PCR testing analysis from swab-based samples, has been fully confirmed. Recognition of multiple variants by the RT-LAMP Duo and Direct Kit is important to ensure public health safety where accurate testing against multiple types of SARS-CoV-2 variants of concern is absolutely crucial in sustaining efforts against pandemic surviliance.<\/p>\r\n<p><b>Methodology:<\/b><\/p>\r\n<p>The <em>in-silico<\/em> analysis was performed by analyzing the NCBI reference sequence of SARS-CoV-2 (NC_045512.2) together with below mentioned whole genome sequences of specific strains deposited in GISAID database EpiCoV:<\/p>\r\n<table class=\"table table-stripped\">\r\n<thead>\r\n<tr>\r\n<th>Accession number<\/th>\r\n<th>Variant<\/th>\r\n<th>WHO classification<\/th>\r\n<\/tr>\r\n<\/thead>\r\n<tbody>\r\n<tr>\r\n<td>EPI_ISL_723044<\/td>\r\n<td>B.1.1.7<\/td>\r\n<td>Alfa<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_825139<\/td>\r\n<td>B.1.351<\/td>\r\n<td>Beta<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_792680<\/td>\r\n<td>P.1<\/td>\r\n<td>Gamma<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_2650470<\/td>\r\n<td>B.1.617.2<\/td>\r\n<td>Delta<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_2631197<\/td>\r\n<td>B.1.427\/B.1.429<\/td>\r\n<td>Epsilon<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_2614193<\/td>\r\n<td>P.2<\/td>\r\n<td>Zeta<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_1563854<\/td>\r\n<td>B.1.525<\/td>\r\n<td>Eta<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_1122452<\/td>\r\n<td>P.3 (version: 2021-04-01)<\/td>\r\n<td>Theta<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_2647531<\/td>\r\n<td>B.1.526<\/td>\r\n<td>Iota<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_1415353<\/td>\r\n<td>B.1.617.1<\/td>\r\n<td>Kappa<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_2536799<\/td>\r\n<td>C.37<\/td>\r\n<td>Lambda<\/td>\r\n<\/tr>\r\n<tr>\r\n<td>EPI_ISL_1259297<\/td>\r\n<td>Breton (hCoV-19\/France\/BRE-IPP04233\/2021)<\/td>\r\n<td>N\/A<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<p>Sequence alignment was performed by utilizing multiple sequence alignment program Clustal Omega (EMBL-EBI) that uses seeded guide trees and HMM profile-profile techniques to generate alignments between three or more genomic sequences. Further visualization of the alignment was performed by using MView (EMBL-EBI).<\/p>\r\n<p>For further wet-lab analysis fragment-specific sequences different from the reference genomic sequences were utilized, as several of the abovementioned new variants exhibit mutations\u2019 similarities in the S and N genes. For this purpose, cDNA for S and N genes specific-fragments where synthetized and after dilution to 1000 copies \/ \u00b5l utilized as a template when setting-up reactions with primer sets utilized in RT-LAMP Duo and Direct Kits.<\/p>\r\n<p>The above-mentioned steps show that Genomtec\u00ae SARS-CoV-2 EvaGreen\u00ae RT-LAMP CE-IVD Duo Kit and oraz Genomtec\u00ae SARS-CoV-2 EvaGreen\u00ae Direct-RT-LAMP CE-IVD Kit successfully detects all of the abovementioned SARS-CoV-2 variants.<\/p>\r\n<p>Genomtec constantly evaluates how newly emerged SARS-CoV-2 variants might impact assay performance by utilizing both <em>in-silico<\/em> as well as wet-lab approach in order to assure customers of the high inclusivity of Genomtec\u00ae SARS-CoV-2 EvaGreen\u00ae RT-LAMP CE-IVD Duo Kit and Genomtec\u00ae SARS-CoV-2 EvaGreen\u00ae Direct-RT-LAMP CE-IVD Kit among SARS-CoV-2 variants of concern.<\/p>\r\n<p>Report has been prepared by: Miron Tokarski, CEO, Genomtec SA<\/p>\r\n<p><strong>Additional information available at: <\/strong><\/p>\r\n<p><strong><a href=\"mailto:bkc.winiarski@genomtec.com\">bkc.winiarski@genomtec.com<\/a><\/strong><\/p>\r\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>RT-LAMP Duo and Direct Kits\u2019 reliability in recognition of identified SARS-CoV-2 significant variants, among them the Breton that has the potential to escape standard RT-PCR testing analysis from swab-based samples, has been fully confirmed. Recognition of multiple variants by the RT-LAMP Duo and Direct Kit is important to ensure public health safety where accurate testing [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":32574,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"footnotes":""},"categories":[5],"tags":[],"class_list":["post-34265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v18.4.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Genetic tests Genomtec SARS-CoV-2 Duo Kit (Cat. no.: GA00B) and Direct-RT-LAMP Kit (Cat. no.: GA00C) detect multiple virus lineages - Genomtec<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/genomtec.com\/en\/2021\/06\/24\/genetic-tests-genomtec-sars-cov-2-duo-kit-cat-no-ga00b-and-direct-rt-lamp-kit-cat-no-ga00c-detect-multiple-virus-lineages\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Genetic tests Genomtec SARS-CoV-2 Duo Kit (Cat. no.: GA00B) and Direct-RT-LAMP Kit (Cat. no.: GA00C) detect multiple virus lineages - Genomtec\" \/>\n<meta property=\"og:description\" content=\"RT-LAMP Duo and Direct Kits\u2019 reliability in recognition of identified SARS-CoV-2 significant variants, among them the Breton that has the potential to escape standard RT-PCR testing analysis from swab-based samples, has been fully confirmed. 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